Microcontact Printing of Proteins on Mixed Self-Assembled Monolayers
نویسندگان
چکیده
Microcontact printing of proteins from an elastomeric stamp has been demonstrated on a limited number of substrates. This work explores the generality of this method of patterning proteins by examining the role of surface wettability of both the substrate and the stamp in microcontact printing. The substrates used in this study consisted of two-component, mixed self-assembled monolayers (SAMs) of alkanethiols on gold presenting -CH3 and polar groups -COOH, -OH, or -(OCH2CH2)6OH. We found that protein adsorbed on a stamp successfully transfers onto a mixed SAM only when the mole fraction of polar functionality on the SAM exceeded a particular threshold. Although the mole fraction of polar groups required was different for each of the three types of mixed SAMs, the advancing water contact angles on these surfaces nearly coincided. Moreover, the minimum wettability of the SAM needed for the transfer of proteins decreased when the wettability of the stamp was decreased. Our findings suggest that the difference in wettability between the surfaces of the stamp and substrate is the dominant parameter that determines the successful microcontact printing of proteins.
منابع مشابه
Ijsing Self-assembled Monolayers to Understand the Interactions of Man- Made Surfaces with Proteins and Cells
Self-assembled monolayers (SAMs) formed on the adsorption of longchain alkanethiols to the surface of gold or alkylsilanes to hydroxylated surfaces are well-ordered organic surfaces that permit control over the properties of the interface at the molecular scale. The abililty to present molecules, peptides, and proteins at the interface make SAMs especially useful for fundamental studies of prot...
متن کاملUsing self-assembled monolayers to understand the interactions of man-made surfaces with proteins and cells.
Self-assembled monolayers (SAMs) formed on the adsorption of long-chain alkanethiols to the surface of gold or alkylsilanes to hydroxylated surfaces are well-ordered organic surfaces that permit control over the properties of the interface at the molecular scale. The ability to present molecules, peptides, and proteins at the interface make SAMs especially useful for fundamental studies of prot...
متن کاملSelective atomic layer deposition of titanium oxide on patterned self-assembled monolayers formed by microcontact printing.
We demonstrate a selective atomic layer deposition of TiO2 thin films on patterned alkylsiloxane self-assembled monolayers. Microcontact printing was done to prepare patterned monolayers of the alkylsiloxane on Si substrates. The patterned monolayers define and direct the selective deposition of the TiO2 thin film using atomic layer deposition. The selective atomic layer deposition is based on ...
متن کاملSubmerged microcontact printing (SmuCP): an unconventional printing technique of thiols using high aspect ratio, elastomeric stamps.
A technique for microcontact printing of thiols in liquid media is presented. Elastomeric poly(dimethyl siloxane) stamps are used to pattern gold surfaces with thiol-based self-assembled monolayers. The liquid (water in this case) has been used as an incompressible support and, advantageously, also acts as a medium in which alkylthiol ink molecules are poorly miscible. Consequently, we have bee...
متن کاملAsymmetric printing of molecules and zeolites on self assembled monolayers.
Microcontact printing (mCP) is used to immobilize dyes and peptides asymmetrically, by a "peptide coupling" reaction, on monolayers of zeolite L crystals in the contact area between the stamp and the surface of the monolayer. Chemically patterned surfaces of monolayers of zeolite L crystals are obtained by using patterned stamps with different ink solutions. Additional printing of functionalize...
متن کامل